SS-31 and Mots-C Recon

Whiynot20026

Whiynot20026

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I have a SS-31 50mg vial. Looks like 3ML recon suggested. I have a Mots-C 20MG vial looks like 2ML suggested. Can anyone that uses these validate these recons.

Thank you,

Bill
 
Those suggested recons are fine. I use the following with no issues recon and no issues with stinging (although not sure if that is a thing with SS31 or MOTSC)"

  • 2.5ml BAC into SS31 50mg as it makes the maths easier.
  • 2ml BAC into MOTS-C 20mg.
 
Whiynot20026 said:


I have a SS-31 50mg vial. Looks like 3ML recon suggested. I have a Mots-C 20MG vial looks like 2ML suggested. Can anyone that uses these validate these recons.

Thank you,

Bill

Click to expand...
Click to expand...

Hi Bill

For SS-31, I make 10 doses of 5 mg from the 50 mg. So the pen lasts 10 days. I’ve switched to 2 ml of 0.9% NaCl (no BA). That makes small volumes of 0.20 ml. No more reactions at the injection site.

I haven't started the MOTS-c again yet. Since the last cycle ended with a huge hard lump on my thigh, I decided to change my strategy.

No more hypotonic BA, I'm switching to 0.9% NaCl. The vial doesn't last long enough for this to be a problem from a bacteriological standpoint. I'll use a pen with a 32G 4mm needle. No more syringes with 8mm at 45°. No more injections in the thighs.

I'll reduce the dose from 5 to 2.5mg.

I have 40 mg vials, which is 16 doses. I'm going to divide them into two cartridges: 8 x 0.30 ml in each, which is 2.4 ml of 0.9% NaCl each. (2.4 in the vial, split in 2x1.2 in cartridges, add 1.2 in each cartridge).

Hypotonicity adds a known problem to the injection. MOTSc adds a possible problem of an allergic reaction, which I hope to mitigate by diluting further and preventing aggregations through higher salinity. Since NaCl brings the pH closer to 7, I'm counting on that as well.

I don't know if you can see it, but I'm enjoying myself. It takes me back to when I was 20 and playing around with cytokines, IgG, HPLC, etc. and radioactive iodine (I can do without that one).
 
eidos said:


Hi Bill

For SS-31, I make 10 doses of 5 mg from the 50 mg. So the pen lasts 10 days. I’ve switched to 2 ml of 0.9% NaCl (no BA). That makes small volumes of 0.20 ml. No more reactions at the injection site.

I haven't started the MOTS-c again yet. Since the last cycle ended with a huge hard lump on my thigh, I decided to change my strategy.

No more hypotonic BA, I'm switching to 0.9% NaCl. The vial doesn't last long enough for this to be a problem from a bacteriological standpoint. I'll use a pen with a 32G 4mm needle. No more syringes with 8mm at 45°. No more injections in the thighs.

I'll reduce the dose from 5 to 2.5mg.

I have 40 mg vials, which is 16 doses. I'm going to divide them into two cartridges: 8 x 0.30 ml in each, which is 2.4 ml of 0.9% NaCl each. (2.4 in the vial, split in 2x1.2 in cartridges, add 1.2 in each cartridge).

Hypotonicity adds a known problem to the injection. MOTSc adds a possible problem of an allergic reaction, which I hope to mitigate by diluting further and preventing aggregations through higher salinity. Since NaCl brings the pH closer to 7, I'm counting on that as well.

I don't know if you can see it, but I'm enjoying myself. It takes me back to when I was 20 and playing around with cytokines, IgG, HPLC, etc. and radioactive iodine (I can do without that one).

Click to expand...
Click to expand...
Thank you very much. This helps immensely.

Bill
 
eidos said:


Hi Bill

For SS-31, I make 10 doses of 5 mg from the 50 mg. So the pen lasts 10 days. I’ve switched to 2 ml of 0.9% NaCl (no BA). That makes small volumes of 0.20 ml. No more reactions at the injection site.

I haven't started the MOTS-c again yet. Since the last cycle ended with a huge hard lump on my thigh, I decided to change my strategy.

No more hypotonic BA, I'm switching to 0.9% NaCl. The vial doesn't last long enough for this to be a problem from a bacteriological standpoint. I'll use a pen with a 32G 4mm needle. No more syringes with 8mm at 45°. No more injections in the thighs.

I'll reduce the dose from 5 to 2.5mg.

I have 40 mg vials, which is 16 doses. I'm going to divide them into two cartridges: 8 x 0.30 ml in each, which is 2.4 ml of 0.9% NaCl each. (2.4 in the vial, split in 2x1.2 in cartridges, add 1.2 in each cartridge).

Hypotonicity adds a known problem to the injection. MOTSc adds a possible problem of an allergic reaction, which I hope to mitigate by diluting further and preventing aggregations through higher salinity. Since NaCl brings the pH closer to 7, I'm counting on that as well.

I don't know if you can see it, but I'm enjoying myself. It takes me back to when I was 20 and playing around with cytokines, IgG, HPLC, etc. and radioactive iodine (I can do without that one).

Click to expand...
Click to expand...
@eidos Can count on you to bring the detailed info -thanks!
 
reta-stacker said:


Radioactive iodine? Mon dieu!

Click to expand...
Click to expand...
With two pairs of gloves, a hood (the chemistry thingy, not on my head) and a Geiger counter, it's manageable.

What felt strange was waiting for the photo of my assays, between two mammograms processed by the hospital lab.
 
eidos said:


I don't know if you can see it, but I'm enjoying myself. It takes me back to when I was 20 and playing around with cytokines, IgG, HPLC, etc. and radioactive iodine (I can do without that one).

Click to expand...
Click to expand...
Being 20 and "cytokines, IgG, HPLC and redioactive iodine" shouldn't be in the sentence, and the verb shouldn't be "playing around with"! I hope it all turned out well!
 
reta-stacker said:


Being 20 and "cytokines, IgG, HPLC and redioactive iodine" shouldn't be in the sentence, and the verb shouldn't be "playing around with"! I hope it all turned out well!

Click to expand...
Click to expand...
To do unpaid research work, it takes a certain amount of passion and having fun, otherwise it'll drive anyone crazy. I searched for cancer but didn't find it. So far, so good.
 
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